This study was made to know the changes of aryl sulfotransferase activity in regenerating rat liver.
Cytosolic, mitochondrial and microsomal aryl sulfotransferase isozymes I,II and III, IV activities were determined in regenerating liver tissue following 70% (median and left lateral lobes) partial hepatectomy in rats for a period of ten days. The activities of these enzymes in serum were measured, the values of the Km and Vmax in these hepatic enzymes were also measured.
The activities of cytosolic, Mitochondrial, and microsomal aryl sulfotransferase I,II in regenerating rat liver tissue showed a significant increase between the second and the third day, the first and the third day, and the twelfth hour and the third day, respectively, following partial hepatectomy.
The activities of aryl sulfotransferase isozymes I,II and III,IV in rat serum showed a significant increase between the twelfth hour and the first day following partial hepatectomy.
The values of Vmax of cytosolic, mitochndrial and icrosomal aryl sulfotransferase isozyme I,II and III,IV in the regenerating liver showed significant increases at the second day following partial hepatectomy However, the values of Km of the these hepatic enzymes did not change.
In View of the above results, aryl sulfotransferase isozymes in regenerating rat liver suggest that these are the enzymes increasing their bisoyntheses in the regenerating stage.
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